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Equipment

A list of MSKC instruments and laboratory material available to registered users. Users can find printed one-page guides located next to the instruments.

Douglas Oryx8 Crystallization Robot

  • Automated crystallization screening using nanodrop dispensing.
  • 0.15  µl to 8 µl per-well dispensing range.
  • Sitting-drop vapour-diffusion using Greiner CrystalQuick low profile, low birefringence, 96-well flat-bottom plates.
  • Other plate settings are possible. Please consult first with the MSKC staff before attempting to change the instrument configuration.
  • Douglas Website.

Scorpion Liquid Handling Robot

  • Art Robbins Instruments Scorpion liquid handler and crystallization screen builder.
  • Easily build n-dimension optimization grids based on your initial crystal hits.
  • Dispense volumes from 1.0 µl to 1,000 µl. 
  • Manufacturer Website.

GE Akta PURE FPLC

  • ​Preparative purification system. Available columns:
  • Ion-exchange: Mono Q 5/50 GL and Mono S 5/50 GL.
  • Size-Exclusion: Superose 6 Increase, Superdex 75 and Superdex 200 Increase.
  • Miscellaneous: HiPrep 26/10 Desalting, HiPrep Butyl FF 16/10 and HiPrep Phenyl FF 16/10.
  • GE Healthcare Website.

Labconco 6L Lyophilizer

  • Freeze dryer system designed for light to moderate loads.
  • Removes a maximum of 4.1 l of water in 24 hours.
  • 8-port manifold drying chamber.
  • Manufacturer Website.

Formulatrix RockImager2 UV-Vis Imager

  • Dual visible light, UV light crystal plate imager, convenient tool to discriminate between protein and salt crystals.​
  • RockMaker software embedded for crystal hit scoring, link to crystallization screen conditions, X-ray diffraction data for scoring and tracking.
  • Accepts all popular crystal plates: SBS-type, Linbro, Greiner, SwissSci.
  • Stage holds up to two crystal trays, 5 minute scan per plate (visible light).
  • Formulatrix Webpage.

Bio-Rad CFX96 Real-Time PCR

  • Real-time protein melt analysis.
  • Thermal shift assay using Sypro Orange readout.
  • We also have the FUNDAMENT and SPECIFIC buffer screens (Jena Bioscience) which you can try if you need help stabilising your protein.
  • Bio-Rad Website.

Tissue Culture Room

  • The TC room is equipped with a Labconco Purifier Class II, Type A2 Biological Safety cabinet for sterile work and a Reach-in-CO2 incubator with two Thermo MaxQ 2000 platform shakers.
  • Growth of mammalian cells at 37℃ in a humidified environment of 5-8 % carbon dioxide.

MaxQ 8000 Orbital Shakers

  • 3 stacked refrigerated shakers each with a 8x clamp capacity.
  • 37℃ cell growth and 16℃ for low temperature induction.
  • 27℃ reserved for baculovirus culture.
  • Thermo Fisher Website.

Sorvall Lynx 4000 Centrifuge

  • 6x 500 ml and 12x 50 ml capacity.
  • Centrifuge max RCF 68,905 x g, max speed 24,000 rpm.
  • Temperature range: -10° to +40°C
  • Fiberlite™ F12-6x500 carbon fiber fixed-angle rotor (speed: max RCF 24,400 x g, max rpm: 12,000 rpm)
  • Fiberlite™ F20-12x50 LEX fixed-angle rotor (max RCF 51,428 x g, max speed: 20,000 rpm)
  • Thermo Fisher Website.

Sonic Dismembrator

  • 700W high-power sonicator, cell disruptor, homogeneizer.
  • Processes 0.2 ml to 1 l.
  • ​Ideal for cell disruption, homogenization, chip/DNA shearing, nano particle dispersion, protein extraction, sonochemistry and soil extraction.
  • Fisher Website.

GE Amersham Imager 600

  • Protein Gel Image acquisition instrument
  • CCD 3.2 Mpixel camera fitted with a large aperture lens
  • Image output: Gray scale 16 bit tif, Color image jpg, Gray scale jpg
  • Image resolution max.: 2816 × 2048, 5.8 Mpixel
  • White Epi light: 470 to 635 nm
  • GE Healthcare Website.

LM20 Microfluidizer High Pressure Shear Homogeneizer

  • LM 20 Microfluidizer High Shear Fluid Processor.
  • High pressure homogeneizer ensures high-yield cell disruption, uniform particle and droplet size reduction with minimal heat denaturation.
  • ​Operates at pressures up to 20,000 psi.
  • Requires a minimum sample size of 14 ml.
  • Microfluidics Webpage.

 

Tissue Culture Essentials

  • A variety of laboratory instruments complement of the TC room, including:
  • A MaxQ 8000 shaker at 27℃ reserved for baculovirus culture (located in the shaker/centrifuge room).
  • One Beckman Z2 cell counter.
  • One Zeiss PrimoVert Microscope.
  • One Thermo Sorvall ST16R centrifuge.
  • Vortexer, water bath, Neubauer cell counting chambers.

Agilent 1260 HPLC

  • Semi-preparative HPLC.
  • ​Upto 100ml/min, 400 bar, 4.6 to 50 mm column i.d.
  • ​C18 column.

SDS-PAGE Gel Electrophoresis Mini Tank

  • SDS-PAGE denaturing protein electrophoresis mini gel tank system.
  • Runs with precast Bolt Bis-Tris Plus (Invitrogen) 4-12 % acrylamide gels: 8 cm x 8 cm (1.0 mm thick).
  • Running buffer: MES SDS to resolve small-, medium-, to large-size proteins (0.3–260 kDa).
  • In-gel detection on staining with PageBlue Protein Staining Solution (Fisher cat. # 24620). PageBlue is colloidal G-250 Coomassie dye, delivers a dynamic range of 5 to 500 ng.
  • PageBlue is a safe, fast application dye (~ 1.5-2 hrs for SDS-PAGE gels), and it can be reused up to three times.
  • Fisher Website.

Rumed 3000 Crystal Incubator

  • Rumed 3000 (Rubarth Apparate Gmbh) incubators set to one single-point temperature value at 8 °C and 12 °C. 
  • The heater and the fan are specially built to minimize variations in temperature, and its operation is essentially vibration-free providing an ideal environment for growing crystals.

Discovery V12 Crystal Imaging Microscope

  • Steroscopic microscope for crystal harvesting.
  • Motorized 12:1 zoom controlled by the external touch panel SYCOP controller.
  • PlanS 1.0x objective offering 8x to 100x magnification.
  • "Cold" LED lightsource and wide base for safe and easy crystal harvesting.
  • Zeiss Website.

NanoDrop 2000c Spectrophotometer

  • Thermo Scientific NanoDrop 2000c UV-Vis spectrophotometer.
  • Quantify and assess purity of DNA, RNA, and protein samples.
  • ​Wavelength range: 190-840 nm. ​Minimum sample size: 0.5 µl.
  • ​Cuvette mode for 1 ml cuvettes (0.5 ml minimum volume). Pathlength: 10, 5, 2, 1 mm. Detection limit: 0.4 ng/μl dsDNA
  • ​Pedestal mode for low volume samples: 0.5-2 µl. Pathlength: 1 mm (auto-ranging to 0.05 mm). Maximum concentration: 15,000 ng/μl (dsDNA). Detection limit: 2 ng/μl dsDNA.
  • Detector: 2048-element linear silicon CCD array.
  • ​Light source: Xenon flash lamp. Absorbance range: 0.02 -300 (10 mm equivalent).

Crystal Cryo-equipment and Crystal Harvesting Tools

  • Complete selection of crystal harvesting tools including cryo pins, loops, casettes and all the necessary cryo-equipment.
  • Standard cryo equipment includes CX-100 shipping dewar, VHC35 storage dewar, LD5 transfer dewar and standard SSRL equipment for use with the SSRL Automated Mounting (SAM) system using SSRL cassettes.

Yeast Protein Production Platform

  • UV-light sterilizable area for yeast culture, manipulation, inoculum/media preparation, plating.
  • Equipped with automated pipettor head, anxillary tools. 

General Lab Equipment

  • A variety of accessory and standard laboratory equipment are available including pH meter, heatblock, stirrers, rockers, rotators, microcentrifuges and microbalance.